Journal: Vaccines

Article Title: Geographical Differences in SARS-CoV-2 Antibody Response Dynamics and Neutralisation Profiles to Mild COVID-19: Lessons from a UK-Uganda Comparison.

doi: 10.3390/vaccines13040336

Figure Lengend Snippet: Figure 4. Correlations between neutralisation titres and SARS-CoV-2 D614G spike, RBD, and nu- cleocapsid IgG binding antibody OD responses stratified by nationality. Figure 4 illustrates the correlations between the SARS-CoV-2 D614G neutralisation titres and IgG optical density (OD) responses targeting spike (S), receptor-binding domain (RBD), and nucleocapsid (N) antigens, strati- fied by the UK and Uganda populations. Scatter plots and correlation coefficients demonstrate the strength and direction of associations for each antigen-specific response across the two populations. The analysis reveals distinct patterns in the relationship between neutralisation titres and D614G antigen-specific IgG responses by nationality.

Article Snippet: ELISA plates (Greiner Bio-One, #655001, Kremsmünster, Austria) were coated with 3 μg/mL of spike or nucleocapsid antigen (R&D Systems, #10474-CV-01M, #10474-CV-01M, Minneapolis, MN, USA), optimised to achieve maximum sensitivity and specificity.

Techniques: Binding Assay